"Bush has no money, he's meeting today with mommy and daddy, and they're working on his campaign," Trump said at the rally in downtown Jacksonville, Fla.
"He's a guy wants to run our country and he can't even run his own campaign. Think of it," he added.
The billionaire business mogul also tried to draw a contrast between Bush's campaign and his, saying he has fewer campaign staff and has spent the least money of all the candidates so far.
"You have all these people, what do you need these people for?" he said. "I put up less money than everyone else, and I'm number one."
Trump is leading in Florida, according to a recent University of North Florida poll of likely Republican primary voters, with 22 percent support.