Yes. I actually heard Single Ladies at dinner last night and people started doing the dance (straight guys, actually) and I heard it on the radio this morning.
Also, "to the left to the left" from Irreplaceable has become a iconic phrase in pop culture, used frequently during everyday conversations.
Crazy in Love speaks for itself.
Destiny's Child hits like Survivor in particular still get people on their feet, motivated to work harder
On a lessor note, the key changes in Love on Top are iconic and everyone worships them.
Absolutely she has made a huge impact